DescriptionUNG Polyclonal antibody specifically detects UNG in Human, Mouse, Rat, Rabbit samples. It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
|This antibody was generated by immunizing rabbit with a synthetic peptide sequence CRHFSKTNELLQKSGKKP corresponding to amino acids 281-298 of human UNG1 (NP 003353.1) and amino acids 290-307 of human UNG2 (NP 550433.1). The peptide sequence used for immunogen is highly conserved between mammalian and invertabrates.|
|Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)|
|PBS containing 0.05% BSA with 0.05% Sodium Azide|
|The synthetic peptide sequence used for immunogen is 100% conserved between UNG1 and UNG2. Thus the antibody recognizes both UNG1 and UNG2. As UNG1 and UNG2 are not resolved as two species in gel electrophoresis (Huag et al., 1998), they are indistinguishable by western blot. The observed molecular weight of UNG (UNG1 and UNG2) on western blots may vary: 26 kDa, 27.5 kDa, 31 kDa, and 38 kDa forms have been described (Bharati et al., 1998; Slupphaug et al., 1993). Please see Kruman et al. (Fig. 1D, 2004) for an example of western blot in rat. This specificity of this antibody has been validated by antisense UNG oligonucleotide (Kruman et al. 2004).|
For Research Use Only
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