uNOS Rabbit anti-Human, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138835
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta tissue.NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.
|NOS1, NOS2, NOS3|
|Synthetic peptide derived from C-terminus of mouse iNOS and nNOS|
|Antigen affinity chromatography|
|PBS with 1% BSA and 0.1% sodium azide; pH 7.4|
|P29474, P29475, P35228|
|HEP-NOS, INOS, NOS, NOS2A|
|-20° C, Avoid Freeze/Thaw Cycles|
|4842, 4843, 4846|
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