Vinculin Monoclonal Antibody (SP117), Invitrogen™

Rabbit Monoclonal Antibody

Supplier:  Thermo Scientific MA516424

Catalog No. PIMA516424

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is heart or small intestine tissue.

Vinculin Monoclonal antibody specifically detects Vinculin in Human samples. It is validated for Immunohistochemistry (Paraffin).

Specifications

• Antigen: Vinculin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: VCL
• Gene Alias: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
• Host Species: Rabbit
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 7414
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Clone: SP117
• Formulation: PBS with 1% BSA and <0.1% sodium azide; pH 7.6
• Gene Accession No.: P18206
• Gene Symbols: VCL
• Immunogen: Synthetic peptide derived from C-terminus of human vinculin protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG