S-arrestin Rabbit anti-Bovine, Mouse, Ovine, Polyclonal, Invitrogen
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA1731
PA1-731 detects recombit bovine and sheep visual arrestin. PA1-731 has been successfully used in immunofluorescence and Western blot procedures. By Western blot this antibody detects an ~57 kDa protein representing recombit bovine and sheep visual arrestin. This antibody also detects a lower molecular weight protein which could correspond to degradation product. The PA1-731 immunizing peptide corresponds to amino acid residues 2-18 from rat visual arrestin. This peptide (Cat. No. PEP-155) is available for use in neutralization and control experiments.Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. In the active state, rhodopsin activates transducin, a GTP binding protein that promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Arrestins are cytosolic proteins that are involved in G protein-coupled receptor (GPCR) desensitization. Arrestin binding to activated GPCRs is phosphorylation dependent and, once bound, uncouple the GPCR from the associated heterotrimeric G proteins. There are currently 4 known mammalian isoforms, beta-arrestin1 (arrestin2), beta-arrestin2 (arrestin3), visual arrestin (arrestin1), and cone arrestin. The beta- isoforms are ubiquitously expressed and are known to interact with acetylcholine and adrenergic receptors. Visual and cone arrestins are found to interact directly with transducin.
|-20° C, Avoid Freeze/Thaw Cycles|
|101105037, 20215, 280922|
|Immunofluorescence, Western Blot|
|PBS with 1mg/mL BSA and 0.05% sodium azide|
|Synthetic Peptide: C E(347) V A T E V P F R L M H P Q P E D(363)|
|Antigen affinity chromatography|
|Bovine, Mouse, Ovine|
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