WT1 Rabbit anti-Human, Mouse, Polyclonal, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Invitrogen PA138865
This antibody is predicted to react with canine, mouse, porcine, rabbit and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is Wilm′s tumor tissue.Wilm’s Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm’s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelioma.
|PBS with 1% BSA and 0.1% sodium azide; pH 7.6|
|WT, AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33, Wilms tumor protein, amino-terminal domain of EWS, last three zinc fingers of the DNA-binding domain of WT1|
|-20° C, Avoid Freeze/Thaw Cycles|
|Synthetic peptide from the C-terminus of human Wilm′s Tumor protein|
|Antigen affinity chromatography|
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