JAML Armenian Hamster anti-Mouse, Clone: eBio4E10 (4E10), eBioscience™ 

Armenian Hamster Monoclonal Antibody

Manufacturer:  Invitrogen 14566682

Catalog No. 50-112-2690

Description

Description: The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation. Applications Reported: This eBio4E10 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. Applications Tested: This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gamma-delta T cell lines, and can provide co-stimulatory signals for cell activation.

Specifications

• Antigen: JAML
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q80UL9
• Gene Alias: AMICA1, CREA7-1, JAML, adhesion molecule, interacts with CXADR antigen 1, AMICA, Gm638
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Mouse

• Applications: Flow Cytometry, Immunoprecipitation
• Clone: eBio4E10 (4E10)
• Conjugate: Unconjugated
• Gene: Jaml
• Format: Liquid
• Gene Symbols: Jaml
• Isotype: IgG
• Quantity: 100 μg
• Storage Requirements: 4° C
• Primary or Secondary: Primary
• Gene ID (Entrez): 270152

For Research Use Only.