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Molecular Probes™ APEX™ Alexa Fluor™ 647 Antibody Labeling Kit Non-distribution product as customer accommodation.

APEX™ Alexa Fluor™ 647 Antibody Labeling Kit

Manufacturer:  Molecular Probes™ A10475

Catalog No. A10475


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Description

Description

The APEX™ Antibody Labeling Kits are our best option for covalently attaching a fluorophore to small amounts of IgG antibody (~10–20 µg). It is ideal for the efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Labeled antibodies are ready for use in imaging or flow cytometry applications in as little as 2.5 hours with very little hands on time.

Important Features of Alexa Fluor™ APEX™ Antibody Labeling Kits:

  • Labeled antibodies typically ready to use in 2.5 hours (~15 minutes hands on time)
  • Designed to label 10–20 µg of IgG
  • Covalent attachment
  • Compatible with contaminating proteins or stabilizers like BSA
  • No columns needed; everything you need is supplied for 5 separate labelings
  • Choose from Alexa Fluor™ 488, 555, 568, 594, and 647 dyes, Oregon Green™ 488 dye, Pacific Blue™ dye, and Biotin-XX.

    Better Results and Workflows With Primary Labeled Antibodies
    A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

    Contaminating Proteins or Protein Stabilizers Are Not a Problem
    Many IgG antibodies are often available only in small quantities and packaged with stabilizing proteins, such as BSA, or other contaminants which can interfere with the amine-reactive labeling reagents. The APEX™ Antibody Labeling Kits avoids this by utilizing a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX™ antibody labeling tip. Contaminants are simply eluted through the tip, prior to applying the amine-reactive label.

    Learn More about Protein and Antibody Labeling
    We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

    We’ll Make a Custom Antibody Conjugate for You
    If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

    For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
  • Specifications

    Specifications

    APEX™ Alexa Fluor™ 647 Antibody Labeling Kit, Far-Red, Fluorescent Detection Method, Alexa Fluor™647 Dye, IgG Antibody TargetMolecule, 650/665nm Excitation/Emission Wavelength, Used for Labeling Chemistry
    Labeling chemistry
    5 Vials reactive fluorescent label (component A), 5 APEX™ antibody labeling tips (component B), 1.8mL wash buffer (Component C), dimethyl sulfoxide (DMSO) (component D), 100μL labeling buffer (component E), 100μL neutralization buffer (compone
    5 rxn(s)
    2 to 6°C
    650nm
    Far-Red
    Alexa Fluor™ 647
    5 Vials reactive fluorescent label (component A), 5 APEX™ antibody labeling tips (component B), 1.8mL wash buffer (Component C), dimethyl sulfoxide (DMSO) (component D), 100μL labeling buffer (component E), 100μL neutralization buffer (compone
    APEX™ Alexa Fluor™ 647 Antibody Labeling Kit
    1 Kit(s)
    665nm
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    For Research Use Only. Not for use in diagnostic procedures.