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Invitrogen™ Apolipoprotein E Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHAPOE

Encompass

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Catalog No. EHAPOE


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Description

Description

The Human Apolipoprotein E (Hu ApoE) ELISA quantitates Hu Apoe in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ApoE. Principle of the method The Human ApoE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13.2.
Specifications

Specifications

Colorimetric Microplate Reader
This ELISA pair antibody detects human ApoE (isoforms ApoE2, ApoE3 and ApoE4).
Biotin
GPCR (G-Protein Coupled Receptors)
P02649
1.5ng/mL to 400ng/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-human Apo E precoated 96-well strip plate, lyophilized recombinant human Apo E standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrat
Plasma, 1 μL; Serum, 1 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
Human
AD2, APO-E, LDLCQ5, LPG
<10%
Human
1.5 ng/mL
Colorimetric
ELISA Kit
96 Assays
Solid-phase sandwich ELISA
HRP
1.64-400 ng/mL
Apolipoprotein E (AD2) ELISA Kit, 96 Assays, 1 Kit, Human Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Apolipoprotein E (AD2) in Samples Using a Microplate Reader
348
RUO
2°C to 8°C
4 hr. 45 min.
Apolipoprotein E (AD2), human ApoE (isoforms ApoE2, ApoE3 and ApoE4)
Cardiovascular Research
<12%
96 Tests
Plasma, Serum, Supernatant
AD2,APO-E,ApoE4,LDLCQ5,LPG
SDS
Documents
Product Certifications
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