BioVision Inc Apoptotic Cell Isolation Kit: Apoptotic Cell Isolation Kit: Rapid & Efficient Kit for Isolating Apoptotic Cells or Removing Dead Cells from Cell Culture or Tissue Preparations using Annexin V/Magnetic Beads. 30 Isolations.
Manufacturer: BioVision Inc K25830
The Apoptotic Cell Isolation Kit provides a simple and efficient means for isolation of apoptotic cells or removal of dead cells from cell culture or tissue preparations using annexin V/magnetic beads (MagBeads). Annexin V is a Ca²ᶧ-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), which is redistributed from the inner to the outer plasma membrane leaflet in apoptotic or dead cells. Once on the cell surface, PS becomes available for binding to annexin V and any of its conjugates. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from living cells. The apoptotic cells bound to the MagBeads adhere to the magnet, while nonapoptotic cells stay in suspension. The separated apoptotic and healthy cells can then be used in a variety of assays to study apoptotic mechanisms and pathways. BioVision charges an Ice pack fee for each order.
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