Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase

Efficiently and reproducibly amplifies long (up to 5 Kb) sequences

Supplier:  Applied Biosystems™ 4398898

Catalog No. 43-988-98

Includes: AmpliTaq Gold 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq Gold 360 PCR Buffer, 25mM M2Cl₂, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq Gold 360 Buffer, 25mM MgCl₂, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of theThermus aquaticusDNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Description

pplied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and optional 360 GC Enhancer, amplifies vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets

• Optimized for broadest range of targets–from everyday to challenging
• Unmatched sensitivity, specificity, and yield
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves highest quality sequencing data
• Less waste, sustainable packaging

See user's manual or package insert for limited label license, and trademark information.

Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Content And Storage: • 25 x 1000 U AmpliTaq Gold 360 DNA Polymerase
  • 100 x 1.5 mL AmpliTaq Gold 360 Buffer, 10X
  • 100 x 1.5 mL 25 mM MgCl₂
  • 100 x 1.5 mL 360 GC Enhancer
  
  Store at -15 to -25°C.
• Format: Tube
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Line: AmpliTaq Gold™
• Quantity: 25 x 1000 Units
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 20000
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Green Features: Sustainable packaging
• Product Type: DNA Polymerase
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.