|5kb or less|
|1 tube (50μL at 5U/μL) of AmpliTaq Gold DNA Polymerase (250 units total); 1 tube (1.5mL) of GeneAmp™ 10X Gold Buffer (150mM Tris-HCl, pH 8.0, 500mM KCl); 1 tube (1.5mL) of 25mM magnesium chloride.|
|Hot Start PCR, PCR, PCR and Real-Time PCR|
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