Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl₂

Purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations

Supplier:  Applied Biosystems™ 4338857

Catalog No. 43-388-57

Includes: Contains AmpliTaq Gold DNA Polymerase, LD (250 units), GeneAmp 10X PCR Gold Buffer, and MgCl₂ Solution.

Description

• Manufacturing process results in highly purified enzyme with lower levels of bacterial DNA
• Minimizes non-target, false positive PCR products when amplifying bacterial sequences
• Chemically modified hot-start technique eliminates biological contamination
• GeneAmp 10X PCR Gold Buffer promotes faster enzyme activation and robust PCR amplification
• Enhanced specificity with time-release PCR is ideal for low-copy target amplification
• AmpliTaq Gold DNA Polymerase, LD improves both yield and success rate for target amplification

• Reliable Results
• Purified enzyme preparation makes advanced PCR techniques fast and easy
• Combined with supplied GeneAmp 10X PCR Gold Buffer, AmpliTaq Gold DNA Polymerase, LD provides a flexible hot-start system
• Suitable for all PCR applications that require the elimination of bacterial DNA contamination

See user's manual or package insert for limited label license and trademark information.

PCR, PCR and Real-Time PCR, Routine PCR

Specifications

• Content And Storage: • 1 x 1000 U AmpliTaq Gold DNA Polymerase, LD
  • 4 x 1.5 mL 10X PCR Gold Buffer
  • 4 x 1.5 mL 25 mM MgCl₂
  
  Store at –15 to –30°C.
• Format: Tube
• Polymerase: AmpliTaq Gold DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Product Type: DNA Polymerase
• Quantity: 1000 Units
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 800
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Line: AmpliTaq Gold™
• Purity or Quality Grade: LD (Low DNA)
• Concentration: 10X
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.