High-Capacity cDNA Reverse Transcription Kit Non-distribution product as customer accommodation.

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2µg total RNA

Manufacturer: Applied Biosystems 4368813

 View all options for this product

Catalog No. 43-688-13

  Check Availability
Add to Cart


    For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
    Format Separate Components
    Includes 2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL)
    Quantity 1000 reactions
    Sample Type RNA
    Storage Requirements Store between -15deg. to -25deg.C
    Temperature (English) Operating 98.6°F
    Temperature (Metric) Operating 37°C

    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2µg of total RNA to single-stranded cDNA in single 20µL reaction. Reactions can be scaled up to 100µL to generate 10µg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

    • Linear target amplification for real-time PCR
    • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
    • Ten-fold greater dynamic range than other kits
    • Optimal reaction temperature: 37°C
    • Final product size: 7kb or less

    • Extensively tested with a variety of templates
    • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

    PCR & Real-Time PCR, Reverse Transcription

  • Description & Specifications

Related Products

Product Content Feedback

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit