High-Capacity cDNA Reverse Transcription Kit Non-distribution product as customer accommodation.

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2µg total RNA

Manufacturer: Applied Biosystems 4368813

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Catalog No. 43-688-13

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    For Use With (Application) cDNA Reverse Transciption, RT-PCR (Endpoint), Real-Time PCR
    Format Separate Components
    Includes 2 × 1mL of 10X RT Buffer; 2 × 1mL of 10X RT Random Primers; 1 × 1mL of 25X dNTP Mix (100mM); 1 × 1mL of MultiScribe Reverse Transcriptase (50U/μL)
    Quantity 1000 reactions
    Sample Type RNA
    Storage Requirements Store between -15deg. to -25deg.C
    Temperature (English) Operating 98.6°F
    Temperature (Metric) Operating 37°C

    The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2µg of total RNA to single-stranded cDNA in single 20µL reaction. Reactions can be scaled up to 100µL to generate 10µg of cDNA from single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

    • Linear target amplification for real-time PCR
    • Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
    • Ten-fold greater dynamic range than other kits
    • Optimal reaction temperature: 37°C
    • Final product size: 7kb or less

    • Extensively tested with a variety of templates
    • Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA

    PCR & Real-Time PCR, Reverse Transcription

  • Description & Specifications

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