Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

Catalog Number	Mfr. No.	No. of Reactions
43-749-67	Applied Biosystems™ 4374967	1,000 Reactions
43-749-66	Applied Biosystems™ 4374966	200 Reactions

Description

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
• Ten-fold greater dynamic range than other kits
• Optimal reaction temperature: 37°C
• Final product size: 7kb or less

Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

PCR & Real-Time PCR, Reverse Transcription

Specifications

• Concentration: 50 U/μL
• Format: Kit
• PCR Method: 1-step RT-qPCR
• Reaction Speed: 120 min.
• Starting Material: RNA
• Volume: 100 μL, 20 μL

• Final Product Type: First-Strand cDNA
• GC-Rich PCR Performance: High
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Technique: Reverse Transcription

For Research Use Only. Not for use in diagnostic procedures.