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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA

$732.00 - $3395.00

Specifications

Concentration 50 U/μL
Final Product Type First-Strand cDNA
Format Kit
GC-Rich PCR Performance High
PCR Method 1-step RT-qPCR
View More Specs

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
Catalog Number Mfr. No. No. of Reactions Price Quantity & Availability  
43-749-67
Encompass
View Documents
Applied Biosystems™
4374967
1,000 Reactions
Each for $3,395.00
Add to cart
 
43-749-66
Encompass
View Documents
Applied Biosystems™
4374966
200 Reactions
Each for $732.00
Add to cart
 
Description

Description

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μL reaction. This kit also includes two tubes of RNase inhibitor (100μL each). Reactions can be scaled up to 100μL to generate 10μg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

  • Linear target amplification for real-time PCR
  • Higher yields and precision than other cDNA synthesis kits—at a fraction of the cost
  • Ten-fold greater dynamic range than other kits
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

Extensively tested with a variety of templates Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.

PCR & Real-Time PCR, Reverse Transcription

Specifications

Specifications

50 U/μL
Kit
1-step RT-qPCR
120 min.
RNA
100 μL, 20 μL
First-Strand cDNA
High
Separate components
Reverse Transcription
Reverse Transcription
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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