Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Supplier:  Applied Biosystems™ 4368577

Catalog No. 43-685-77

Includes: SYBR™ Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components

Description

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix.

• Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient single-tube format
• Power SYBR™ Green PCR Master Mix contains all of the components (excluding the template and primers) for superior SYBR™ Green reagent-based real-time PCR
• The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer increased sensitivity
• Contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG to minimize carryover contamination
• Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error and sample evaporation
• Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions
• Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifications

• Content And Storage: The 2x mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. Contains 1 X 1 mL vial, sufficient for 40 reactions at 50 μL. Store at -20°C.
  
  Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
• Format: Tube
• PCR Method: qPCR
• Reaction Speed: Standard
• Label or Dye: SYBR Green
• Product Line: SYBR™
• Purity or Quality Grade: LD (Low DNA)
• Shipping Condition: Wet Ice
• For Use With (Application): Gene Expression
• No. of Reactions: 40 Reactions
• Volume: 1 x 1 mL

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• Polymerase: AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
• For Use With (Equipment): 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
• Passive Reference Dye: ROX (Pre-mixed)
• Product Type: Real Time PCR SYBR Master Mix
• Quantity: 1 x 1 mL
• Sufficient For: 40 Reactions of 50 μL
• Concentration: 2X
• Sample Type: DNA (Genomic), cDNA

For Research Use Only. Not for use in diagnostic procedures.