SYBR™ Fast Green Master Mix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Designed to obtain a fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or efficiency

Manufacturer: Applied Biosystems 4385612

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Catalog No. 43-856-12

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Required Accessories : Template and primers


    For Use With (Application) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
    Includes 2X mixture of SYBR Green I Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components
    Quantity 1 x 5mL

    • Speed—Real-time PCR results in as fast as 35 minutes
    • Sensitive—Detect very low copies of target
    • Specific—Minimize primer-dimer and nonspecific amplification
    • Reproducible—Consistent amplification across a wide dynamic range

    • Fast SYBR Green Master Mix contains all of the components, (except template and primers) in a convenient 2X master mix.
    • Includes the following components in an optimized buffer: AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing nonspecific product formation and enabling reactions to be set up at room temperature
    • SYBR Green I dye to enable detection of double-stranded DNA
    • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
    • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
    • Passive internal reference based on proprietary ROX™ dye to enable increased precision

    Fast Real-Time PCR, Gene Expression Analysis & Genotyping, PCR & Real-Time PCR, Real Time PCR (qPCR), Real Time PCR-Based Gene Expression Profiling

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

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