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Applied Biosystems™ SYBR™ Green PCR Master Mix Non-distribution product as customer accommodation.

Everything needed for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format.

Manufacturer:  Applied Biosystems™ 4344463

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Catalog No. 43-444-63


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Specifications

Description

Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

  • Premixed components stored at 2° to 8°C significantly reduce assay setup time
  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience:
  • Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
Description & Specifications

Specifications

Real time PCR (qPCR), real time PCR-based gene expression profiling
1 x 1mL
Primer-Probe Compatability: Unlabeled Oligos
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Standard
SYBR™ Green PCR Master Mix in a 1mL vial. Sufficient reagents provided for 40 reactions based on a 50μL reaction volume.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
DNA (Genomic), cDNA

Safety and Handling

ShelfLife : Guaranteed minimum shelf life is 60 days.

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