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Applied Biosystems™ SYBR™ Select Master Mix For CFX Non-distribution product as customer accommodation.

A cost-effective solution for real-time PCR applications.

Manufacturer:  Applied Biosystems™ 4472942

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Catalog No. 44-729-42

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For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.



  • Specific - minimize primer-dimer and non-specific amplification
  • Reproducible and sensitive - consistent amplification across a wide dynamic range
  • Bright - contains SYBR™ GreenER™ dye for maximum brightness
  • Carry-over contamination control - contains heat-labile UDG
  • For use with the Bio-Rad CFX96™ Touch or CFX384™ Touch systems
  • Standard and fast cycling parameters

Formulated for Maximum Specificity:
  • SYBR™ Select Master Mix for CFX contains all the components needed for your real-time PCR reactions, except the template and primers, in a convenient 2X concentration premix
  • The master mix includes AmpliTaq™ DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity

Obtain Reproducible Results Across a Wide Dynamic Range:
  • SYBR™ Select Master Mix for CFX is specially formulated to provide robust results from 100ng to 0.1pg cDNA per reaction and can reliably detect a single copy of genomic DNA.
    • Contains SYBR™ GreenER™ Dye and Heat-labile UDG:
    • SYBR™ GreenER™ dye is less inhibitory to PCR than SYBR™ Green I dye, resulting in brighter signals
    • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control

    Order Info

    Shipping Conditions: Wet Ice

Description & Specifications


Purity or Quality Grade: UP (Ultra Pure)
2X mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, and optimized buffer components. Contains 1 x 5mL bottle, sufficient for 500 reactions at 20μL.
Store at 2° to 8°C.
Detection Method: SYBR; PCR Method: qPCR
cDNA, DNA (Genomic)
Real time PCR (qPCR), real time PCR-based gene expression profiling
1 x 5mL
Primer-Probe Compatability: Unlabeled Oligos
Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™
Fast, Standard

Safety and Handling

ShelfLife : Guaranteed minimum shelf life is 60 days.

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