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Applied Biosystems™ TaqMan™ Gene Expression Master Mix Non-distribution product as customer accommodation.

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Manufacturer:  Applied Biosystems™ 4370048

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Catalog No. 43-700-48


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Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Template and primers

Specifications

Description

TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis, pathogen detection and viral load quantification,co-amplification of two targets, as well as specific detection of gene family members
TaqMan™ Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase, UP (Ultra Pure) for hot start activation and improved detection of bacterial targets, a blend of dNTPs with dTTP/dUTP and Uracil-DNA Glycosylase (UDG) to minimize carry-over PCR contamination, and a passive internal reference based on proprietary ROX™ dye for superb precision on Applied Biosystems™ real-time PCR instruments.

  • Optimized 2X mix that contains all of the components, excluding the template and primers, for sensitive detection down to one copy of target
  • Extended benchtop stability provides consistent results for automated liquid-handling systems, making it the choice for high-throughput sample processing, overnight or over the weekend
Description & Specifications

Specifications

ROX (Pre-mixed)
Store at 2°C to 8°C.
Detection Method: Primer-Probe Detection, PCR Method: qPCR
cDNA, DNA (Genomic)
1 tube (1mL) 2X TaqMan Gene Expression Master Mix sufficient for 40 reactions. TaqMan Gene Expression Master Mix contains AmpliTaq Gold™ DNA Polymerase (Ultra Pure), Uracil-DNA glycosylase, dNTPs (with dUTP), ROX™ Passive Reference, and optimized buffer components.
1 x 1mL
Pharma and biopharma, real time PCR (qPCR), real time PCR-based gene expression profiling, target and lead identification and validation
7300 System, StepOnePlus™, Standard Mode, 7000 System, 7900HT System, 7500 System, StepOne™, Standard Mode

Safety and Handling

Recommended Storage : Store at 2° to 8°C.

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