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R&D Systems™ AQUApure Tetra-Ub Chains (K11-linked) Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Supplier: R&D Systems™ UC45025
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Description
The AQUApure Tetra-Ub Chains (K11-linked) Protein is derived from E. coli. The AQUApure Tetra-Ub Chains (K11-linked) Protein has been validated for the following applications: Bioactivity.Specifications
P0CG47 | |
7314 | |
25μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening. | |
UBB | |
Unconjugated | |
E. coli-derived |
Bioactivity | |
Theoretical M.W.: 34kDa | |
E. coli-derived human Tetra-Ubiquitin protein | |
Tetra-Ubiquitin | |
Ubiquitin chains vary in length, linkage, and function. K11-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°C for 20 minutes prior to gel electrophoresis. | |
Human | |
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain. Ub-AQUA analysis: K11: 98.87% K63: 0.82% K33: 0.11% K48: 0.11% K6 : 0.08% All other linkages ≤ 0.01% |
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