R&D Systems™ AQUApure Tetra-Ub Chains (K33-linked), Carrier-Free Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ UC103025

Catalog No. UC103025

Description

The AQUApure Tetra-Ub Chains (K33-linked) Protein is derived from E. coli. The AQUApure Tetra-Ub Chains (K33-linked) Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P0CG47
• Gene ID (Entrez): 7314
• Quantity: 25μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Gene Symbol: UBB
• Conjugate: Unconjugated
• Expression System: E. coli-derived

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Theoretical M.W.: 34kDa
• Source: E. coli-derived human Tetra-Ubiquitin protein
• Common Name: Tetra-Ubiquitin
• Biological Activity: Ubiquitin chains vary in length, linkage, and function. K33-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°C for 20 minutes prior to gel electrophoresis.
• Species: Human
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain. Ub-AQUA analysis: K33: 95.08% K48: 2.69% K63: 0.90% K11: 0.71% All other linkages ≤ 0.38%