Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas. Applications Reported: This sl6arg antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry. Applications Tested: This sl6arg antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 20 µg/mL. This sl6arg antibody has also been tested by immunocytochemistry of fixed and permeabilized human cells and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Arginase-1 (ARG1) catalyzes the hydrolysis of L-arginine into ornithine and urea, a critical step for the urea cycle. Defects in ARG1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia. There are several isoforms of mammalian arginase. The type I isoform, is a cytosolic enzyme and expressed predomitly in the liver as a component of the urea cycle. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells.
|Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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