ASH2L Monoclonal Antibody (4C5), Active Motif™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-3343	Active Motif 61360	10 μg
50-199-3342	Active Motif 61359	100 μg

Description

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.

Specifications

• Antigen: ASH2L
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene Alias: ASH 2; ASH2; ash2 (absent, small, or homeotic)-like; ash2 (absent, small, or homeotic)-like (Drosophila); ASH2 LIKE; ASH2 like histone lysine methyltransferase complex subunit; ASH2 like protein; ASH2 like, histone lysine methyltransferase complex subunit; Ash2l; ASH2L1; ASH2L2; ash2-like; ASH2-like protein; BOS_23936; Bre 2; Bre2; LOC100338600; Set1/Ash2 histone methyltransferase complex subunit ASH2
• Gene Symbols: ASH2L
• Isotype: IgG2a
• Purification Method: Protein G
• Product Type: Antibody

• Clone: 4C5
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Human
• Gene Accession No.: Q9UBL3
• Gene ID (Entrez): 9070
• Immunogen: This antibody was raised against a recombinant protein corresponding to the N-terminal region of human ASH2L.
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: ASH2L