BioVision Inc Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit: High-Throughput Suitable Assay for Measuring ALT/SGPT Activity in Serum, Tissues, Cells etc. Simple, Sensitive and Reliable. Detection Limit: 10 mU. 100 Assays
Manufacturer: BioVision Inc K753100
AST also known as Glutamate-oxaloacetate transaminase (GOT) is a transaminase (EC 18.104.22.168) similar to the more liver specific alanine transaminase (ALT). Although commonly included clinically as part of a diagnostic liver function test, AST has a broader clinical utility since it may also be elevated in diseases affecting other organs, such as the heart or muscles in myocardial infarction, also in acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease, musculoskeletal diseases and trauma. It catalyzes the reaction: Aspartate + ⍺-Ketoglutarate ⇌ Oxaloacetate + Glutamate Diagnostically, it is almost always measured in units/liter (U/L). In this Kit, an amino group is transferred of from aspartate to ⍺-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). Detection limit of 10 mU per well.
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