Axygen™ AxyPrep Mag™ DyeClean Kits Available on GSA/VA Contract for Federal Government customers only.

Scalable dye terminator clean-up simplified

Manufacturer: Axygen Scientific MAGDYECL250

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Catalog No. 14-223-165

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    For Use With (Application) Removes excess dye terminator chemistries
    For Use With (Equipment) ABI PRISM 3730, 3730xl, 3700, 3130, 31030xl and 3100, MegaBACE
    Format 96 and 384-well plate
    No. of Reactions 25000 to 35000 Preps
    Product Type Kit
    Quantity 250mL

    Excess dye terminator clean-up post sequencing reaction clean-up is an essential step prior to sequencing. The carryover of excess dye into sequencing results in dye blobs which leads to overwhelming noise and failed calls of sequenced bases and potentially leads to the failure of the sequencing run. AxyPrep Mag DyeClean utilizes a unique paramagnetic bead-based sequencing purification system optimized for the clean-up of excess dye terminator post sequencing reaction in Sanger sequencing. The protocol mainly consists of binding, washing and elution steps, and can be performed directly in the thermal cycling plate and requires no centrifugation or filtration.

    • Saves time and money: Significant BigDye savings without incurring extra cost and processing time with unneeded BigDye signal enhancer buffer, while maintaining high QV20+ scores
    • High signal-to-noise ratio resulting in long reads and high QV20+ scores
    • Efficient removal of various excess dye terminator chemistries including: BigDye versions 1.1 and 3.1 and DYEnamic ET
    • Suitable for the clean-up of sequencing reaction of different Sanger sequencing based platforms: ABI Prism 3730, 3730xl, 3700, 3130, 3130xl and 3100- GE Healthcare MegaBACE
    • Simple and easy sequencing reaction clean-up process: 25 minutes/96 samples
    • Manual and automation friendly
    • Scalable: tube, 96 and 384 well microplate formats
    • No centrifugation or filtration required

  • Description & Specifications

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