Thermo Scientific B-PER Reagent (in Phosphate Buffer)
Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.
Manufacturer: Thermo Scientific 78266
Perform efficient, gentle lysis and extraction of E.coli and other bacterial cells with Thermo Scientific B-PER Bacterial Protein Extraction Reagents.These easy-to-use cell lysis reagents are nonionic detergent solutions that effectively disrupt cells and solubilize native or recombinant proteins without denaturation. Certain formulations include DNase I and lysozyme enzyme supplements to improve yield of large molecular weight proteins that are difficult to purify. B-PER Reagents are compatible with the addition of standard protease inhibitors and with downstream protein purification methods, such as GST purification and His-tagged immobilized metal affinity chromatography (IMAC).
Ready to use B-PER Reagents provide one-step E.coli cell lysis by a mild, nonionic detergent (proprietary) in your choice of Tris or phosphate buffer formulations
Fast and simple just add B-PER Reagent to a bacterial pellet, shake for 10 minutes and recover soluble proteins after pelleting the cell debris
Excellent yields recover both soluble and insoluble recombinant protein from bacterial lysates and purify inclusion bodies to near-homogeneous levels
Flexible B-PER Reagents are suitable for any scale of protein extraction and are available in phosphate and 1X and 2X Tris formulations, with and without enzymes
Compatible completely compatible with addition of protease inhibitor cocktails, and resulting protein extract can be used in protein assays, typical affinity purification methods (e.g., GST, 6xHis) and other applications
Convenient, ready-to-use formats:
- B-PER and B-PER II (2X) Reagents are free of enzymatic components
- B-PER Reagent (in phosphate buffer) is an amine-free formulation for direct compatibility with labeling and crosslinking
- B-PER with Enzymes and B-PER Direct with Enzymes include lysozyme and DNase I for improved lysis and recovery
Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
|Store at room temperature.|
|Detergent Solution, Cell Lysis Buffer|
|B-PER Bacterial Protein Extraction Reagent (in Phosphate Buffer)|
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