B7-2/CD86 Antibody (C86/1146) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2477780.2MG

Catalog No. NBP247778B

Description

B7-2/CD86 Monoclonal antibody specifically detects Antigen in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting.

Specifications

• Antigen: B7-2/CD86
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 1 ug/mL, Immunohistochemistry-Paraffin : 2 - 4 ug/ml, Flow (Cell Surface) 1:10-1:1000, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready, Knockout Validated
• Gene Alias: Activation B7-2 antigen, B70, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86 antigen, CD86 molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
• Host Species: Mouse
• Molecular Weight of Antigen: 70 kDa
• Quantity: 0.2 mg
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cell Cycle and Replication, Dendritic Cell Markers, Diabetes Research, Immunology, Myeloid derived Suppressor Cell
• Gene ID (Entrez): 942
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Clone: C86/1146
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: CD86
• Immunogen: Recombinant human full-length CD86 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ