Invitrogen™ B7-H3 (CD276) Monoclonal Antibody (M045)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547649

Catalog No. PIMA547649

Description

Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE Native or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

CD276 is a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.

Specifications

• Antigen: B7-H3 (CD276)
• Classification: Monoclonal
• Concentration: 0.25 mg/mL
• Formulation: PBS with 1 mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q5ZPR3
• Gene Symbols: CD276
• Immunogen: Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: M045
• Conjugate: Unconjugated
• Gene: CD276
• Gene Alias: 4Ig-B7-H3; 6030411F23Rik; AU016588; B7 homolog 3; B7h3; B7-H3; B7RP-2; CD276; CD276 antigen; CD276 molecule; CD276 molecule tv1; costimulatory molecule; PSEC0249; sB7 H3; sB7H3; sCD276; soluble B7 H3; soluble B7H3; soluble CD276; UNQ309/PRO352
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 80381
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid