B7-H4 Monoclonal Antibody (H74), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 16594982

Catalog No. 50-146-45

Description

Description: The H74 monoclonal antibody was generated and reacts with human B7-S1, also known as B7-H4 and B7x. Cross reactivity of this antibody to other proteins has not been determined. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. While it is reported that BTLA is a counter receptor for B7S1, further studies are needed to definitely determine the B7-H4 ligand. H74 stains human B7-H4 transfected cells and not peripheral blood cells. Exact expression pattern of B7-H4 has not been fully characterized. Applications Reported: This H74 antibody has been reported for use in flow cytometric analysis. Applicability of this antibody in in vitro assays for blocking or activation has not been determined. Applications Tested: This H74 antibody has been tested by flow cytometric analysis of transient hB7-H4 transfected cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment.

B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).

Specifications

• Antigen: B7-H4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: Q7Z7D3
• Gene Symbols: Vtcn1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79679
• Content And Storage: 4° C
• Form: Liquid

• Applications: Control, Flow Cytometry, Functional Assay
• Clone: H74
• Conjugate: Functional Grade
• Gene: Vtcn1
• Gene Alias: B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7s1; B7x; BC032925; EMBL:AAH95842.1}; FLJ22418; immune costimulatory protein B7-H4; PRO1291; Protein B7S1; RGD1311204; RP11-229A19.4; t cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; UNQ659/PRO1291; VCTN1; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; Vtcn1; vtcn1 {ECO:0000312
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.