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BCL6 Rat anti-Mouse, PE, Clone: BCL-DWN, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 12545380

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Catalog No. 50-112-2325


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Description

Description

Description: This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, which is also known as B-cell lymphoma 6. A member of the POZ family of transcriptional repressors, Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79-kDa protein is essential for germinal center B cell proliferation and survival. Moreover, during B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt ft.s lymphoma, and non-Hodgkin ft.s lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1. Applications Reported: This BCL-DWN antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BCL-DWN antibody has been tested by flow cytometric analysis of splenocytes isolated from sheep red blood cell-immunized mice. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Bcl-6 proto-oncogene product (a Kruppel-type zinc-finger protein) is mainly expressed in normal germinal center B cells and related lymphomas. Bcl-6 is involved in chromosome rearrangements at 3q27 in non-Hodgkin ft.s lymphomas and Bcl-6 rearrangements have been detected in 33%-45% of diffuse large B cell lymphomas. Bcl-6 has been detected immunohistochemically in follicular lymphomas, diffuse large B cell lymphomas, Burkitt ft.s lymphomas and in nodular, lymphocyte predomit Hodgkin ft.s disease.
Specifications

Specifications

BCL6
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P41183
B-cell lymphoma 6 protein; BCL-6; Zinc finger protein 51; LAZ-3 protein; BCL-5; Zinc finger and BTB domain-containing protein 27; ZNF51; ZBTB27; LAZ3; BCL5; BCL6
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
BCL-DWN
PE
Bcl6
Liquid
Bcl6
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12053
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