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BD Difco™ Leptospira Enrichment EMJH

Used with Leptospira Medium Base EMJH in cultivating Leptospira. BD™ Difco™ Leptospira Enrichment EMJH is a lyophilized media additive. The solution contains albumin, polysorbate 80 and additional growth factors for Leptospira.

Manufacturer:  BD 279510

Catalog No. DF0795-73-1

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The basal medium and enrichment are prepared according to the formulations described by Ellinghausen and McCullough, as modified by Johnson and Harris (by replacing rabbit serum medium with polysorbate 80-albumin). Leptospira Medium EMJH is recommended for the clinical isolation of Leptospira from clinical specimens and environmental samples. The Enrichment media contains albumin, polysorbate 80 and additional growth factors for Leptospira.

  • Leptospira Medium Base plus Enrichment shows good culture response to:
    Leptospira interrogans (serotype australis) 23605,
    Leptospira interrogans (serotype canicola) 23470
    Leptospira kirschneri (serotype grippotyphosa) 23604
  • Add 100mL of Enrichment to a solution of 900mL purified water plus 2.3g Medium Base
  • Water content: 89.4%
  • Vapor Pressure (at 20°C): 23.0 hPa (17mm Hg)
  • Soluble in water

In 1886, Adolf Weil described the first recognized leptospiral infections in humans. These cases were caused by Leptospira icterohaemorrhagiae and the disease was subsequently named Weil’s Disease. Leptospirosis is a zoonotic disease, with infection usually resulting from direct or indirect exposure to the urine of leptospiruric animals.



6 x 100mL
730 Days
Medium to dark amber
Leptospira Enrichment
Albumin, polysorbate 80 and additional growth factors for Leptospira
Leptospira medium base EMJH
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