BDNF Recombinant Polyclonal Antibody (19HCLC), Invitrogen™

Rabbit Recombinant Polyclonal Antibody

Supplier:  Thermo Scientific 710306

Catalog No. PI710306

Description

This antibody is predicted to react with non-human primate, rat and rabbit based on sequence homology. Intact IgG appears on a non-reducing gel as ∽150 kDa band and upon reduction generating a ∽25 kDa light chain band and a ∽50 kDa heavy chain. Recombit rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombit monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombit antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombit rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer′s and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Antigen: BDNF
• Classification: Recombinant Polyclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P23560, P21237
• Gene Symbols: BDNF
• Immunogen: Recombit protein corresponding to amino acids 129–247 of human BDNF
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: 19HCLC
• Conjugate: Unconjugated
• Gene: BDNF
• Gene Alias: Brain-derived neurotrophic factor, Abrineurin
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 12064, 627
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid