beta Tubulin Monoclonal Antibody (2-28-33), Active Motif™

Mouse Monoclonal Antibody

Supplier:  Active Motif 61158

Catalog No. 50-199-3195

Description

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

Specifications

• Antigen: beta Tubulin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene Accession No.: P04350, P07437, Q13509, Q13885, Q3ZCM7, Q9BUF5, Q9H4B7
• Gene Symbols: TUBB, Tubb1, Tubb2a, Tubb3, Tubb4a, Tubb6, TUBB8
• Immunogen: This beta-tubulin antibody was raised against sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
• Quantity: 10 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 2-28-33
• Conjugate: Unconjugated
• Gene: Tubb1
• Gene Alias: 2310057H16Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA631M21.2; BB220206; beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4; beta-4 tubulin; beta-4-tubulin; beta-5; beta-5 tubulin; beta5-tubulin; betaIII-tubulin; Beta-tubulin class-III; Beta-tubulin class-V; Beta-tubulin class-VI; Beta-tubulin isotype I; BOS_13459; BOS_17166; BOS_21644; BOS_22279; BOS_7641; c(beta)7 tubulin; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; CH242-247L10.4; class I beta tubulin; Class I beta-tubulin; class IIa beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta tubulin; class V beta-tubulin; CSCSC1; dJ40E16.7; dystonia 4, torsion (autosomal dominant); DYT4; FEOM3; HSA10p15 beta-tubulin 4Q; HsT1601; hypothetical protein LOC550458; I79_000194; I79_012097; LOC100347858; LOC100358689; LOC100761889; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; M40 antibody; Neuron-specific class III beta-tubulin; OK/SW-cl.56; O
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 10381, 10382, 203068, 347688, 7280, 81027, 84617
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid