Invitrogen™ Blood Group H Type 2 Monoclonal Antibody (19-OLE)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA135386

Catalog No. 01-671-441

Description

19-OLE reacts with H type 2 antigen, the basis of the ABO blood group system, involving three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. It is expressed on endothelial cells, epithelial cells and granulocytes. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas.

The H antigen is a precursor to the ABO blood groupantigens, present in people of all common blood types. The Bombay phenotype (hh) does not express antigen H on red blood cells, and therefore this type will also lack A or B antigens, similar to the O blood group. However, unlike O group, the H antigen is absent, hence the individuals produce isoantibodies to antigen H as well as to both A and B antigens. If they receive blood from someone with O blood group, the anti-H antibodies will bind to the H antigen on the red blood cells of the donor blood and destroy the RBCs bycomplement-mediated lysis. Therefore, people with Bombay phenotype can receive blood only from otherhhdonors, although they can donate as though they were type O. Some individuals with the blood group A1 may also be able to produce anti-H antibodies due to the complete conversion of all the H antigen to A1 antigen. Production of the H antigen, or its deficiency in the Bombay phenotype, is controlled at the H locus on chromosome 19. The H locus contains threeexonsthat span more than 5kbof genomic DNA, and encodes thefucosyltransferasethat produces the H antigen on RBCs. The H antigen is a carbohydrate sequence withcarbohydrateslinked mainly to protein (with a minor fraction attached toceramidemoiety).

Specifications

• Antigen: Blood Group H Type 2
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: P16442
• Gene Symbols: ABO
• Immunogen: Mucin isolated from a mucinous colonic adenocarcinoma.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Clone: 19-OLE
• Conjugate: Unconjugated
• Gene: ABO
• Gene Alias: ABH; BG-H; BG-H1
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 28
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov