BRCA1 Monoclonal Antibody (6B4), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA123164

Catalog No. PIMA123164

Description

MA1-23164 detects BRCA1 in Human and Mouse samples. MA1-23164 has been successfully used in chromatin immunoprecipitation, immunohistochemistry, immunocytochemistry, immunofluorescence, Immunoprecipitation and Western Blot procedures. The predicted molecular weight is 220 kDa. A suggested positive control for this product is HBL100. MA1-23164 immunogen corresponds to BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748. Store at 4°C short term. For extended storage, store at -20°C or below, avoiding freeze/thaw cycles.

BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

Specifications

• Antigen: BRCA1
• Classification: Monoclonal
• Concentration: 2.75 mg/mL
• Gene: BRCA1
• Gene Alias: Breast Cancer 1, Breast Cancer 1 Early Onset, Breast Ovarian Cancer Susceptibility, IRIS, Papillary Serous Carcinoma Of The Peritoneum, PSCP, RNF53
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12189, 672
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: 6B4
• Conjugate: Unconjugated
• Gene Accession No.: P38398, P48754
• Gene Symbols: BRCA1
• Immunogen: BRCA1 protein fragment expressed in E. coli corresponding to amino acids 341-748.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1 κ