BioVision Inc BrdU Cell Proliferation Assay Kit: BrdU Cell Proliferation Assay Kit: Easy & Sensitive BrdU based Colorimetric Assay to Measure Cell Proliferation in response to growth factors, cytokines, mitogens and nutrients etc. Kit Size: 200 & 1000 Assays.
Manufacturer: BioVision Inc K306200
5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Biovision's BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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