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Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Colorimetric Non-distribution product as customer accommodation.

Contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Manufacturer:  Invitrogen™ 8800659945

Catalog No. 50-112-5060


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Alert:

Contains Sodium azide, formaldehyde, and hydrogen peroxide


Includes: BrdU Fixation Buffer
BrdU Antigen Retrieval Solution (10X)
BrdU Blocking Solution
Anti-BrdU Biotin (clone BU20A) : 10 µg
Avidin-HRP
3, 3'-Diaminobenzidine (DAB) Substrate Solution: Reagent A (DAB Chromogen), Reagent B (DAB Diluent), and Dropper Bottle (for storage and application of working DAB solution)

Description

Description

  • Cells are labeled in vitro or in vivo with 5-bromo-2 ft.-deoxyuridine (BrdU), which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle
  • Following fixation and antigen-retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP and DAB substrate reaction
Specifications

Specifications

Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections
50 tests
Chimpanzee, Human, Macaque, Mouse, Rat
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