BrdU Monoclonal Antibody (MoBU-1), FITC, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA112686

Catalog No. 01-674-037

Description

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.08% sodium azide
• Host Species: Mouse
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry
• Clone: MoBU-1
• Conjugate: FITC
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Immunogen: Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles, store in dark
• Form: Liquid
• Target Molecule: Chemical