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BrdU Mouse anti-Chemical, PE, Clone: BU20A, eBioscience™ Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 12507142

Encompass

Catalog No. 12-507-142


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Description

Description

This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment. For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (cat. 00-5525) and protocol. Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Staining Buffer Set (cat. 00-5523) or the BrdU Staining Buffer Set (cat. 00-5525) and protocol. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

BrdU Monoclonal antibody specifically detects BrdU in Chemical samples. It is validated for Flow Cytometry
Specifications

Specifications

BrdU
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Mouse
100 Tests
Primary
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
BU20A
PE
5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Affinity chromatography
RUO
Chemical
Antibody
IgG1 κ
SDS
Documents
Product Certifications
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