BrdU Recombinant Mouse Monoclonal Antibody, AbFlex™, Active Motif™

Mouse Recombinant Monoclonal Antibody

Supplier:  Active Motif 91269

Catalog No. 50-199-3789

Description

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Specifications

• Antigen: BrdU
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: FRA10B
• Gene Symbols: FRA10B
• Immunogen: This antibody was raised against Bromodeoxyuridine conjugated to BSA (Bovine Serum Albumin).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunofluorescence
• Concentration: 1 mg/mL
• Formulation: 140mM HEPES, 32mM sodium acetate with 30% glycerol, 70mM NaCl and 0.035% sodium azide; pH 7.5
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 2432
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid