BrdU Monoclonal Antibody (Bu20a), Active Motif™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-3292	Active Motif 61274	10 μg
50-199-3291	Active Motif 61273	100 μg

Description

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Specifications

• Antigen: BrdU
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene ID (Entrez): 2432
• Immunogen: This BrdU antibody was raised against Bromodeoxyuridine conjugated to BSA and recognizes BrdU.
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: FRA10B

• Clone: Bu20a
• Applications: Immunohistochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Multi-species, Mouse, Rat
• Gene Alias: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
• Gene Symbols: FRA10B
• Isotype: IgG1
• Purification Method: Protein A
• Product Type: Antibody