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ErbB3 (HER-3) Mouse anti-Human, PerCP-eFluor™ 710, Clone: SGP1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$151.00 - $335.00

Specifications

Antigen ErbB3 (HER-3)
Clone SGP1
Target Species Human
Conjugate PerCP-eFluor™ 710
Isotype IgG1
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-245-934 Non-distribution product as customer accommodation. View Documents Invitrogen
46655541
25 Tests Each for $151.00
Add to cart
 
50-245-935 Non-distribution product as customer accommodation. View Documents Invitrogen
46655542
100 Tests Each for $335.00
Add to cart
 
Description

Description

Description: This SGP1 monoclonal antibody reacts with human c-ErbB3, which is also known as HER3. This epidermal growth factor (EGF) receptor family member is expressed on normal human tissues, particularly on neurons and cells of the gastrointestinal tract. c-ErbB3 is overexpressed in a variety of cancers, including breast, ovary, and gastrointestinal carcinomas. Upon binding its ligand heregulin beta-1, c-ErbB3 is activated by heterodimerization with ErbB2 or EGFR1. Dependent on PI3K signaling, c-ErbB3 mediates tumor cell invasion, intravasation, and metastasis. Expression of this cell surface receptor has been used as a prognostic marker for breast cancer. The SGP1 antibody has been reported to stimulate anchorage-independent growth of breast cancer cell lines. Applications Reported: This SGP1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SGP1 antibody has been pre-titrated and tested by flow cytometric analysis of the MCF7 cell line. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).

Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. c-erbB-3/HER-3 is the third member of the type I family of growth factor receptors. It binds to ligands in the heregulin family. C-erbB-3 is over-expressed in a variety of tumors inculding breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents.
Specifications

Specifications

ErbB3 (HER-3)
Human
IgG1
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
SGP1
PerCP-eFluor™ 710
Flow Cytometry
Primary
Monoclonal
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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