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c-MAF Mouse anti-Human, Mouse, eFluor® 660, Clone: sym0F1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 50985580

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Catalog No. 50-112-4559



Description

Description

Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems. In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific. More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4. c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells. It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well. Applications Reported: This sym0F1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This sym0F1 antibody has been tested by intracellular staining followed by flow cytometric analysis of TCR-activated mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.

Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors. The leucine-zipper domain is involved in the interaction with LRPICD. There are two forms of human c-maf mRNA, c-maf-long and c-maf-short. It is identified in the genome of the acute transforming avian retrovirus AS42. c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet , p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE). It regulates Th2 differentiation and lineage-specific hematopoiesis. c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages. Chromosomal aberration involving maf is found in some forms of multiple myeloma. It is expressed in myeloma cell lines and resting monocytes/macrophages.
Specifications

Specifications

c-Maf
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O75444, P54843
2810401A20Rik, A230108G15Rik, AW047063, c-maf
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse
Flow Cytometry
sym0F1
eFluor™ 660
MAF
Liquid
MAF
IgG2b, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
17132, 4094
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