MilliporeSigma Calbiochem DNA Methyltransferase Inhibitor II, SGI-1027
A cell-permeable quinoline compound that is shown to inhibit human DNMT1 (IC50 = 6 and
Manufacturer: MilliporeSigma 26092110MG
DescriptionA cell-permeable quinoline compound that is shown to inhibit human DNMT1 (IC50 = 6 and 12.5µM, respectively, with Hemimethylated DNA or Poly(dI-dC) as the substrate), murine DNMT3a and 3b (IC50 = 8 and 7.5µM, respectively, with Poly(dI-dC) as the substrate), as well as CpG DNA methylase M. Sss I (IC50 = 13.5 and 16µM, respectively, with Poly(dI-dC) as the substrate and 75 or 150nM AdoMet) of mycoplasma Spiroplasma sp. strain MQ1, presumably by competing with AdoMet (S-adenosyl-L-methionine , SAM) for the enzyme's cofactor binding site. Shown to be 2-, 3-, and 5-fold more effective than Decitabine (Cat. No. 189825), respectively, in reactivating p16 mRNA (2.5µM for 7 days), MLH1 mRNA (2.5µM for 7 days), and p16 protein expression (1.0 or 2.5µM for 12 days) in human colon carcinoma RKO cultures by reducing CpG island hypermethylation found in the promoter regions of TSGs (tumor suppressor genes) without inducing genome-wide hypomethylation or caspase-3 activation.
|88% by HPLC|
|Protect from Light|
|Pale yellow powder|
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