MilliporeSigma™ Calbiochem™ L3MBTL3 MBT Domain Blocker, UNC1215

A cell-permeable pyrrolidinyl-piperidinyl-benzamide that effectively competes against H4K20Me2 peptide for L3MBTL3 binding by targeting the first two L3MBTL3 MBT domains ( K _d = 120nM in binding studies using recombinant 3MBT fragment), while exhibiting much reduced potency against L3MBTL1-H4K20Me1, 53BP1-H4K20Me2, MBTD1-H4K20Me1, or L3MBTL4-H2AK36Me1 interaction (IC₅₀ ≥2μM; [peptide]

Supplier:  MilliporeSigma™ 5.00557.0001

Catalog No. 50-055-70001

Description

A cell-permeable pyrrolidinyl-piperidinyl-benzamide that effectively competes against H4K20Me2 peptide for L3MBTL3 binding by targeting the first two L3MBTL3 MBT domains ( K _d = 120nM in binding studies using recombinant 3MBT fragment), while exhibiting much reduced potency against L3MBTL1-H4K20Me1, 53BP1-H4K20Me2, MBTD1-H4K20Me1, or L3MBTL4-H2AK36Me1 interaction (IC₅₀ ≥2μM; [peptide] = 150nM). Shown to increase GFP-L3MBTL fusion nucleus mobility (10nM to 1μM) in HEK293 transfectants. X-ray structural analysis reveals two 3MBT are bridged together by two UNC1215 molecules in a reciprocal fashion.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

Specifications

• Color: Off-white
• Target Proteins: Kme binding ligand of L3MBTL3
• Formula Weight: 529.7g/mol
• Content And Storage: Hygroscopic, Protect from light
• Quantity: 10 mg

• Description: 98% by HPLC
• Molecular Formula: C₃₂H₄₃N₅O₂
• Solubility: DMSO
• Form: Powder