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BioVision IncSupplier Diversity Partner Calpain Activity Fluorometric Assay Kit: Calpain Activity Assay Kit: Rapid, Simple & Convenient Assay to Measure Calpain Activity. Includes Calpain Inhibitor, Z-LLY-FMK. Detection Method: Fluorescence. 100 Assays. DFS Item

Manufacturer:  BioVision IncSupplier Diversity Partner K240100

Calpain Activity Fluorometric Assay Kit: Activation of calpain is involved in many forms of physiological and pathological processes (e.g., apoptosis). The Calpain Activity Assay Kit provides optimized buffers and reagents for a convenient measurement of calpain activity. The Extraction Buffer provided with the kit specifically extracts cytosolic proteins. The Extraction Buffer also prevents auto-activation of calpain during the extraction procedure. The kit detects only activated calpain in cytosol upon treatment of cells with inducers. The fluorometric assay is based on the detection of cleavage of calpain substrate Ac-LLY-AFC. Ac-LLY-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by calpain, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorecence plate reader. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.

Catalog No. 50-111-9641


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