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Molecular Probes™ CaptAvidin™ Agarose (Sedimented Bead Suspension)

CaptAvidin™ Agarose (Sedimented Bead Suspension)

Manufacturer:  Molecular Probes™ C21386

Encompass

Catalog No. C21386


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Description

Description

CaptAvidin™ biotin-binding protein conjugated to agarose is designed for use in affinity chromatography. CaptAvidin™ biotin-binding protein contains a nitrated tyrosine in its biotin-binding site. This chemical modification permits dissociation of the avidin-biotin complex under milder conditions than the traditional avidin-biotin complex. At pH 4.0, CaptAvidin™ biotin-binding protein binds biotin tightly with a Ka of ∼109M-1. This association can then be reversed at pH 10, allowing complete dissociation of the avidin-biotin complex. This is in contrast to the harsh conditions (8 M guanidine hydrochloride, pH 1.5) needed to dissociate the avidin-biotin complex.
Specifications

Specifications

Agarose
Wet Ice
Safety and Handling

Safety and Handling

SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.