Invitrogen™ Cardiotin Monoclonal Antibody (SR-4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA106310

Catalog No. MA106310

Description

MA1-06310 detects cardiotin in human and porcine samples. MA1-06310 has sucessfully been used in Western blotting and immunohistochemistry. By Western blotting, it detects a ~300 kDa protein representing cardiotin from. The MA1-06310 immunogen is cardiotin purified from mouse spleen cells.

Cardiotin is a recently discovered, high molecular weight protein complex (~300 kDa) located in the longitudinal SR of cardiomyocytes. The cardiotin complex exists as 60 kDa and 100 kDa subunits. Both subunits contain the same amino-terminal 14 amino-acid sequence, and show high homology to human skeletal muscle alpha-actin. The tetrameric configuration of the cardiotin protein complex suggests that it exists as a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. Immunofluorescence studies colocalize cardiotin with SERCA 2a ATPase in the longitudinal sarcoplasmic reticulum (SR), although cardiotin is not expressed in junctional SR with SERCA 2.

Specifications

• Antigen: Cardiotin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Immunogen: Cardiotin purified from mouse spleen cells.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Western Blot
• Clone: SR-4
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Purified
• Regulatory Status: RUO
• Target Species: Human, Pig
• Product Type: Antibody
• Isotype: IgM

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov