Cas9 Monoclonal Antibody (7A9-3A3), Active Motif™

Mouse Monoclonal Antibody

Supplier:  Active Motif 61578

Catalog No. 50-199-3515

Description

Cas9 is a RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering. Staphylococcus aureus derived Cas9 has a number of properties that make it advantageous for genome editing, including its small size, high efficiency, nickase activity, and apparent specificity. SaCas9 principally recognizes an NNGRRT protospacer adjacent motif (PAM) and cleaves target DNA at rates comparable to SpCas9 (Cas9 derived from S. pyogenes) with an ideal spacer lengths of 24 to 20 nucleotides. In comparison to SpCas9, SaCas9 is smaller (1368 AA versus 1053 AA) and generates indels at a comparable rate when directed against target DNA with the mutually permissive NGGRRT PAM. This makes it the preferred enzyme for all-in-one delivery of Cas9 and multiple gRNA expression cassettes with Adeno-associated vectors.

Specifications

• Antigen: Cas9
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 30% glycerol and 0.035% sodium azide; pH 7.5
• Gene Accession No.: P55211
• Gene Symbols: CASP9
• Immunogen: This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
• Quantity: 10 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Clone: 7A9-3A3
• Conjugate: Unconjugated
• Gene: CASP9
• Gene Alias: APAF3; APAF-3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; cas9; CASP 9; CASP9; CASP-9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; Caspase-9 precursor; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; CRISPR-Cas9; CTD; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; OTTHUMP00000002324; PPP1R56; protein phosphatase 1, regulatory subunit 56; RNCASP9; RP11-265F14.3
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 842
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid