BioVision Inc Caspase-1 Colorimetric Assay Kit: Caspase-1/ICE Activity Colorimetric Assay Kit: Simple & Convenient colorimetric Assay to Measure Caspase-1/ICE Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Manufacturer: BioVision Inc K11125
Activation of ICE-family proteases/caspases initiates apoptosis or other cellular processes in mammalian cells. The Caspase-1/ICE Colorimetric Protease Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate YVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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